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KMID : 0981820080280010016
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Korean Journal of Laboratory Medicine
2008 Volume.28 No. 1 p.16 ~ p.23
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Dissemination of IMP-1 and OXA Type -Lactamase in Carbapenem-resistant Acinetobacter baumannii
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Sung Ji-Youn
Kim Ji-Myung
Koo Sun-Hoe
Kwon Kye-Chul
Park Jong-Woo
Kim Yeon-Sook
Shin Kyeong-Seob
Kim Jong-Wan
Ko Chi-Seon
Shin So-Youn
Song Jong-Hoon
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Abstract
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Background : Acinetobacter baumannii is an aerobic, gram-negative, glucose-nonfermenting bacterium, which has emerged as a serious opportunistic pathogen. In recent years, the increasing instance of carbapenem-resistant A. baumannii producing metallo- -lactamases (MBLs) or OXAtype -lactamases is causing a serious clinical problem. In this study, we investigated the prevalence of Ambler class A, B, and D -lactamases and their extended-spectrum derivatives in carbapenem-resistant A. baumannii isolates.
Methods : A total of 31 consecutive, non-duplicate, carbapenem-resistant A. baumannii were isolated from three university hospitals in the Chungcheong province of Korea. The modified Hodge and inhibitor-potentiated disk diffusion tests were conducted for the screening of carbapenemase and MBL production, respectively. PCR and DNA sequencing were performed for the detection of β-lactamase genes. We also employed the enterobacterial repetitive intergenic consensus (ERIC)- PCR method for the epidemiologic study.
Results : Twenty-three of 31 isolates harbored blaOXA-2 (51.6%), blaOXA-23 (22.6%), blaIMP-1 (48.4%), and blaVIM-2 (3.2%). All of the OXA-2-producing strains also evidenced MBLs. The strains that harbored blaOXA-23 were isolated only in hospital C, and only in a limited fashion. The ERIC-PCR pattern of the five OXA-23 strains indicated that the isolates were closely related in terms of clonality. The six strains producing IMP-1 isolated from hospital A were confirmed to be identical strains.
Conclusions : A. baumannii strains harboring IMP-1 or OXA-type -lactamases are currently widely distributed throughout the Chungcheong province of Korea. The most notable finding in this study was that a blaOXA-2-producing A. baumannii harboring MBL, which has not been previously reported, can also lead to outbreaks. (Korean J Lab Med 2008;28:16-23)
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KEYWORD
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A. baumannii, MBL, OXA-2
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